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The combined working solutions of analytes in the desired concentration range were prepared by appropriate dilution of CS and QC stock solutions with methanol/water (1/1, v/v)

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In a healthy dog, the pancreas, intestines, liver, and kidneys are all needed to properly absorb and store B12

The relative gene expression was calculated using the 2 CT method by Ribosomal protein L13a ( RPL13 ) gene, and the fold change was expressed by 2 CT

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Acknowledgements The authors declare that no specific funding or external assistance was received for this research, and no acknowledgements are made